Tenascin C, Cell Signaling Technology, 12221

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Li et al. Nature Communications, 2020. Li et al. evaluated this antibody for the detection of endogenous mouse Tenascin-C (TNC) by Western blotting using control and CRISPR-Cas9-generated TNC-knockout (TNC-KO) 4T1-Atg5KO cells. A strong TNC band at approximately 250 kDa was detected in control cells, whereas the corresponding signal was markedly reduced in TNC-KO cells (Figure 3D), but signal was still present. However, note that this antibody has been discontinued. Based on the available validation data, we have assigned this antibody a three-star rating, but we highly encourage other scientists to share their observations and experiences with this antibody.