AP2M1, Abcam, ab75995

I am sharing a pAbmAbs-generated literature review of KO-validated antibodies, here citing Yuan et al., Science Advances, 2020. Yuan et al. validated the AP2M1 antibody ab75995 for the detection of endogenous human AP2M1 by Western blotting using wild-type (WT) and AP2M1-knockout (AP2M1−/−) 293T cells. Following subcellular fractionation, AP2M1 was detected predominantly in the cytoplasmic fraction of WT cells, whereas no corresponding AP2M1 signal was observed in AP2M1−/− cells (Figure 5E), supporting the specificity of the antibody for AP2M1. Based on the available validation data, we have assigned this antibody a five-star rating, but we highly encourage other scientists to share their observations and experiences with this antibody.