PRMT4, Cell Signaling Technology, 12495

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Yin et al. Nature communications 2021. Yin et al. validated this antibody for the detection of endogenous human Protein Arginine Methyltransferase 4 (PRMT4) by Western blot analysis of whole-cell lysates (WCLs) derived from MCF7 cells in which PRMT4 was knocked out using PRMT4-targeting lentiCRISPR constructs (sg-1 and sg-2). Using the antibody at a dilution of 1:1000, no PRMT4 band was detected in the MCF7 KO cell lysates (Supplementary Figure 1d). Based on the available data, we have assigned a five-star rating but we highly encourage other scientists to share their observations.