PRMT3, Abcam, ab191562

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Yin et al. Nature communications 2021. Yin et al. validated this antibody for the detection of endogenous human Protein Arginine Methyltransferase 3 (PRMT3) by Western blot analysis of whole-cell lysates (WCLs) derived from MCF7 cells in which PRMT3 was knocked out using PRMT3-targeting lentiCRISPR constructs (sg-1 and sg-2). Using the antibody at a dilution of 1:10000, a faint PRMT3 band was detected in the sg-1 KO cell lysates but no band observed in sg-1 KO (Supplementary Figure 1c) Based on the available data, we have assigned a four-star rating but we highly encourage other scientists to share their observations.