Netrin-1, R&D Systems, AF6419

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Netrin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Netrin-1 encoded by NTN1.

The collection of antibodies was tested using MCF7 WT and NTN1 KO cells in the applications WB (WT and KO cell culture medium/10 µg protein) and IP (WT culture medium) according to YCharOS standard protocols (see reference).

In Western blot (1:30 antibody dilution), AF6419 detected several faint bands, one of which was more prominent in WT culture medium at approximately 65 kDa. No band was detected following IP (2 ug Ab used).