LRP-1, Developmental Studies Hybridoma Bank, 2C6

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Prolow-density lipoprotein receptor-related protein 1 (LRP-1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human LRP-1 encoded by LRP1.

The collection of antibodies was tested using HAP1 WT and LRP1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:4 ab dilution) 2C6 detected no band. No band was detected following IP (2 µg Ab used). In IF (1:10 ab dilution), comparable staining intensities were observed in both KO and WT cell lysate.

We highly encourage other scientists to share their observations.