Huntingtin, Developmental Studies Hybridoma Bank, MW5-S

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Huntingtin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human Huntingtin encoded by HTT.

The collection of antibodies was tested using DMS35 WT and HTT KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:10 Ab dilution) MW5-S detected similar pattern of bands in both genotypes, none of which were specific to WT cell lysate. No band was detected following IP (µg Ab used). In IF (1:15 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.