Annexin A11, Aviva Systems Biology, ARP87950_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Annexin A11 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 15 commercial research antibodies against human Annexin A11 encoded by ANAX11.

The collection of antibodies was tested using HeLa WT and ANAX11 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) ARP87950_P050 detected a band of appr. 54 kDa in WT lysate, whit no corresponding band observed in KO lysate. Two bands of lower molecular weight were observed in WT. In the following IP (1 µg Ab used), only a weak band was observed compared to the corresponding band input extract (10% of starting material) and immunodepleted extract (10 % of total unbound fraction), showing that the antibody does not efficiently immunoprecipitate the antigen. In IF (1:400 Ab dilution) comparable staining intensities were observed in both KO and WT cells for both PFA and methanol fixation. We highly encourage other scientists to share their observations