alpha-1-antitrypsin, Proteintech, 82918-1-RR

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Alpha-1-antitrypsin (A1AT) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 18 commercial research antibodies against human Alpha-1-antitrypsin (A1AT) encoded by SERPINA1

The collection of antibodies was tested using HepG2 WT and SERPINA1 KO cells in the applications WB (WT and KO culture media and cell lysate/30 µg protein), IP (WT culture medium) and flow cytometry (KO and WT cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 ab dilution) 82918-1-RR detected a band of appr. 60 kDa in WT culture medium with no corresponding band observed in KO culture medium. The same pattern was observed when using cell lysate for WB. A band was detected following IP (2 µg Ab used), this band had same intensity compared to bands in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction), showing that the antibody does not deplete the antigen from cell lysate. Flow cytometry using 82918-1-RR (1:8000 ab dilution) showed a right-shifted fluorescence intensity distribution in WT cells compared with KO cells. The KO population overlapped completely with the secondary-only control. Overlap between the two populations were still observed.