alpha-1-antitrypsin, Aviva Systems Biology, ARP96977_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Alpha-1-antitrypsin (A1AT) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 18 commercial research antibodies against human Alpha-1-antitrypsin (A1AT) encoded by SERPINA1

The collection of antibodies was tested using HepG2 WT and SERPINA1 KO cells in the applications WB (WT and KO culture media and cell lysate/30 µg protein), IP (WT culture medium) and flow cytometry (KO and WT cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) ARP96977_P050 detected a band of appr. 60 kDa in WT culture medium with no corresponding band observed in KO culture medium. When using cell lysate for WB, no band specific to WT cell lysate was detected. No band was detected following IP (2 µg Ab used). Flow cytometry using ARP96977_P050 (1:5000 ab dilution) showed overlapping fluorescence intensity distributions for WT and KO cells, with minimal visible shift between the two populations.