ARID1A, Proteintech, 83733-3-RR

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human AT-rich interactive domain-containing protein 1A (ARID1A) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human AT-rich interactive domain-containing protein 1A (ARID1A).

The collection of antibodies was tested using HAP1 WT and ARID1A KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:5000 ab dilution) 83733-3-RR detects a WT-specific signal at the expected high-molecular-weight region (~245 kDa), which is absent in the knockout sample. The immunoreactive signal appears as a cluster of closely migrating bands rather than a single distinct band. A faint band was detected following IP (2 µg Ab used). In IF (1:300 ab dilution), comparable staining was observed in WT and KO cells.