ARID1A, Cell Signaling Technology, 35499

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human AT-rich interactive domain-containing protein 1A (ARID1A) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human AT-rich interactive domain-containing protein 1A (ARID1A).

The collection of antibodies was tested using HAP1 WT and ARID1A KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 35499 detects a WT-specific signal at the expected high-molecular-weight region (~245 kDa), which is absent in the knockout sample. The immunoreactive signal appears as a cluster of closely migrating bands rather than a single distinct band. Furthermore, faint bands of lower molecular weight were observed in both genotypes. A band was detected following IP (2 µg Ab used), this band was more intense compared to band in input extract (6% of starting material). A band was also detected in immunodepleted extract (6% of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:200 ab dilution), a more staining was observed in WT cells compared to KO cells exhibiting background staining.