Cathepsin B, Proteintech, 31399-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Cathepsin B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 11 commercial research antibodies against human Cathepsin B encoded by CTSB.

The collection of antibodies was tested using HAP1 WT and CTSB KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1:1000 ab dilution) 31399-1-AP detected a band of appr. 30 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Furthermore, bands of higher molecular weight were observed in both genotypes. A band was detected following IP (2 µg Ab used) targeting mature Cathepsin B, this band was markedly more intense compared to band in input extract (4% of starting material) and no band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:300 ab dilution), clear staining was observed in WT cells compared to KO cells showing no stain.