DJ-1, Aviva Systems Biology, ARP51267_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human DJ-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human DJ-1 encoded by PARK7.

The collection of antibodies was tested using HEK293T WT and PARK7 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) ARP51267_P050 detected a band of appr. 24 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Furthermore, intense bands of higher molecular weight were observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:200 ab dilution), staining was observed in WT cells whereas KO cells exhibited background staining.