DJ-1, ABclonal, A0987

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human DJ-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human DJ-1 encoded by PARK7.

The collection of antibodies was tested using HEK293T WT and PARK7 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) A0987 detected a band of appr. 24 kDa in WT cell lysate only. A band was detected following IP (2 µg Ab used), this band was more intense compared to band in input extract (6% of starting material). A band was also detected in immunodepleted extract (6% of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:1500 ab dilution), staining was observed in WT cells with no background staining observed in KO cells.