VEGFR1, Thermo Fisher Scientific, MA5-32045

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Vascular endothelial growth factor receptor 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Vascular endothelial growth factor receptor 1 encoded by FLT1.

The collection of antibodies was tested using A-375 WT and VPS35 KO cells in the applications WB (WT and KO culture medium and cell lysate/20 µg protein) and IP (WT culture medium) according to YCharOS standard protocols (see reference).

In WB (1:5000 ab dilution) MA5-32045 detected a double band of appr. 150 kDa in WT culture medium with no corresponding band observed in KO culture medium, however an intense band of higher molecular weight was observed in both genotypes. In WB on culture medium MA5-32045 did not detect a band specific to WT cell lysate. No band was detected following IP (2 µg Ab used).