VEGFR1, Abcam, ab32152

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Vascular endothelial growth factor receptor 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Vascular endothelial growth factor receptor 1 encoded by FLT1.

The collection of antibodies was tested using A-375 WT and VPS35 KO cells in the applications WB (WT and KO culture medium and cell lysate/20 µg protein) and IP (WT culture medium) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab32152 detected a double band of appr. 150 kDa in WT culture medium with a faint corresponding band observed in KO culture medium. In WB on culture medium ab32152 did not detect a band specific to WT cell lysate. No band was detected following IP (2 µg Ab used).