PCB, Aviva Systems Biology, ARP59606_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human pyruvate carboxylase and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human pyruvate carboxylase encoded by PC.

The collection of antibodies was tested using HAP1 WT and PC KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) ARP59606_P050 did not detect a band specific to WT cell lysate. Only a faint band was detected following IP (2 µg Ab used). In IF (1:500 ab dilution) clear staining was observed in WT cells with minimal background staining observed in KO cells.