SCARB2, Thermo Fisher Scientific, 703037

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Scavenger receptor class B member 2 (SCARB2) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human SCARB2.

The collection of antibodies was tested using MCF7 WT and SCARB2 KO cells in the applications WB (WT and KO cell lysates), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 703037 detected a band of appr. 75 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band of interest was detected following IP.
In IF (1:500 ab dilution) a more intense staining was observed in WT cells when permeabilized with Saponin compared to permeabilization with Triton X-100, where comparable staining was observed in both genotypes.