SCARB2, Novus Biologicals, NBP3-22186

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Scavenger receptor class B member 2 (SCARB2) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human SCARB2.

The collection of antibodies was tested using MCF7 WT and SCARB2 KO cells in the applications WB (WT and KO cell lysates), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) NBP3-22186 detected a band of appr. 75 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP.
In IF (1:200 ab dilution) comparable staining intensity was observed in both KO and WT cells.