Beclin-1, Aviva Systems Biology, ARP86769_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Beclin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 19 commercial research antibodies against human Beclin-1 encoded by BECN1.

The collection of antibodies was tested using HeLa WT and BECN1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and flow cytometry (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) ARP86769_P050 did not detect a band specific to WT cell lysate. A band was detected following IP (2 µg Ab used), this band was more intense compared to band in input extract (6% of starting material). A faint band was also detected in immunodepleted extract (6% of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate.