Galectin-1, Thermo Fisher Scientific, MA5-35566

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Galectin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Galectin-1 encoded by LGALS1.

The collection of antibodies was tested using HAP1 WT and LGALS1KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 ab dilution) MA5-35566 detected a faint band of appr. 14 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. An intense smear was observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:100 ab dilution), intense staining was observed in WT cells whereas some background staining was observed in KO cells.