Galectin-1, Thermo Fisher Scientific, MA5-32779

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Galectin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Galectin-1 encoded by LGALS1.

The collection of antibodies was tested using HAP1 WT and LGALS1KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 ab dilution) MA5-32779 detected a band of appr. 14 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was detected following IP (2 µg Ab used), this band was more intense compared to band in input extract (4% of starting material). A band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:2000 antibody dilution), faint staining was observed in most WT cells, whereas no staining was detected in KO cells.