Galectin-1, R&D Systems, MAB1152

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Galectin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Galectin-1 encoded by LGALS1.

The collection of antibodies was tested using HAP1 WT and LGALS1KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:250 ab dilution) MAB1152 detected a band of appr. 14 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Multiple faint bands (>10) of higher molecular weight were observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:500 ab dilution), comparable staining intensity was observed in KO and WT cells.