Galectin-1, Aviva Systems Biology, ARP58491_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Galectin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Galectin-1 encoded by LGALS1.

The collection of antibodies was tested using HAP1 WT and LGALS1KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:100 ab dilution) ARP58491_P050 detected a faint band of appr. 14 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. An intense smear was observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:250 ab dilution), a more intense staining was observed in WT cells while KO cells also exhibit background staining.