Galectin-1, Abcam, ab192842

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Galectin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Galectin-1 encoded by LGALS1.

The collection of antibodies was tested using HAP1 WT and LGALS1KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:100 ab dilution) ab192842 detected a band of appr. 14 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Furthermore, faint bands of higher molecular weight were observed in both genotypes. A band was detected following IP (2 µg Ab used), this band had same intensity compared to bands in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction), showing that the antibody does not deplete the antigen from cell lysate. In IF (1:100 ab dilution), a more intense stain was observed in WT cells compared to KO cells.