KIF5A, Abcam, ab322900

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Kinesin Family Member 5A (KIF5A) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human KIF5A

The collection of antibodies was tested using U-87 MG WT and KIF5A KO cells in the applications WB (WT and KO cell lysates/60 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab322900 detected a band of appr. 117 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to band in input extract (2% of starting material) and no band was detected in immunodepleted extract (2% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:500 ab dilution), comparable staining intensity was observed in WT and KO cells.