NRF2, Abcam, ab62352

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human nuclear factor erythroid 2-related factor 2 (NRF2) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 17 commercial research antibodies against human nuclear factor erythroid 2-related factor 2 (NRF2) encoded by NFE2L2.

The collection of antibodies was tested using HCT116 WT and NFE2L2 KO MG-132 treated cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and flow cytometry (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab62352 detected a band of appr. 95 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Faint bands of lower molecular weight were observed in WT cell lysate. No protein was detected in the unbound fraction during IP (2 µg antibody used); however, the immunoprecipitated fraction was not included. Flow cytometry using ab62352 (1:7270 ab dilution) showed overlapping fluorescence intensity distributions for WT and KO cells, with no visible shift between the two populations.