TREM2, GeneTex, GTX637387

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Triggering receptor expressed on myeloid cells 2 (TREM2) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Triggering receptor expressed on myeloid cells 2 encoded by the TREM2 gene

The collection of antibodies was tested using THP-1 WT and TREM2 KO cells in the applications WB (WT and KO cell medium/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) GTX637387 detected a band of appr. 31 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Intense bands of higher molecular weight were observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:1000 ab dilution), comparable staining intensity was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.