CDK5, Thermo Fisher Scientific, MA5-31987

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Cyclin-dependent kinase 5 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Cyclin-dependent kinase 5 encoded by CDK5.

The collection of antibodies was tested using HAP1 WT and CDK5 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA5-31987 detected a band of appr. 33 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Immunoprecipitation using 2 μg of antibody failed to detect a putative target band. In IF (1:1000 ab dilution), clear staining was observed in WT cells with no background staining observed in KO cells.