CDK5, Aviva Systems Biology, ARP38525_T100

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Cyclin-dependent kinase 5 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Cyclin-dependent kinase 5 encoded by CDK5.

The collection of antibodies was tested using HAP1 WT and CDK5 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ARP38525_T100 detected an intense band of appr. 33 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Fainter bands of higher molecular weight were observed in both genotypes. Immunoprecipitation using 2 μg of antibody failed to detect a putative target band. In IF (1:1000 ab dilution), comparable staining intensity was observed in KO and WT cells.