CDK5, Abbexa, abx422613

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Cyclin-dependent kinase 5 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Cyclin-dependent kinase 5 encoded by CDK5.

The collection of antibodies was tested using HAP1 WT and CDK5 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) abx422613 detected a band of appr. 33 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Furthermore, multiple bands (>10) of both higher and lower molecular weight were observed in both genotypes. Immunoprecipitation using 2 μg of antibody failed to detect a putative target band. In IF (1:1000 ab dilution), comparable staining intensity was observed in KO and WT cells.