Syntenin-1, Thermo Fisher Scientific, MA5-35685

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Syntenin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Syntenin-1 encoded by SDCBP.

The collection of antibodies was tested using HAP1 WT and SDCBP KD cells in the applications WB (WT and KO cell lysates/30 µg protein and culture medium/ 30 µg protein ), IP (WT cell lysate and culture medium) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) on both cell lysate and culture medium, MA5-35685 detected a band of appr. 32 kDa in WT with no corresponding band observed in KD. A band was detected following IP (2 µg Ab used) on cell lysate, this band was markedly more intense compared to band in input extract (6% of starting material) and no band was detected in immunodepleted extract (6% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. IP on culture medium produced the same result. In IF (1:800 ab dilution), WT cells showed more staining compared to KD cells.