Rab6A, Novus Biologicals, NBP2-32386

Our team performed a comprehensive review of the scientific literature to identify studies using antibodies against human Rab6A, followed by extraction and evaluation of available data according to pAbmAbs rating criteria.

YCharOS conducted a systematic head-to-head comparison of 13 commercially available antibodies against Rab6A across multiple applications

Antibodies were tested by WB using lysates (40 µg protein) from HAP1 WT cells and HAP1 Rab6A KO cells, which may express a truncated lower molecular weight variant. These cell lines were also used for IF. In addition, antibodies were evaluated by IP using HAP1 cell lysates (1 mg protein). All experiments were performed according to YCharOS standard protocols (see reference).

In WB (1:200 antibody dilution), NBP2-32386 detects a very faint band at the expected size (~24 kDa) in WT HAP1 cell lysate with no corresponding band in lysate from Rab6A KO cells. In addition several very intense background bands are detected in both lysates.

In IP, the antibody (2 µg) markedly enriches Rab6A, as detected by WB using the Rab6A-specific antibody NBP3-13701. However, the target band remains detectable in the unbound fraction, indicating incomplete immunoprecipitation.

In IF, the antibody (1:50 dilution) resulted in a clear staining of HAP1 WT with some background staining observed in Rab6A KO cells.

We highly encourage other scientists to share their observations.