Rab6A, Novus Biologicals, NBP1-33110

Our team performed a comprehensive review of the scientific literature to identify studies using antibodies against human Rab6A, followed by extraction and evaluation of available data according to pAbmAbs rating criteria.

YCharOS conducted a systematic head-to-head comparison of 13 commercially available antibodies against Rab6A across multiple applications

Antibodies were tested by WB using lysates (40 µg protein) from HAP1 WT cells and HAP1 Rab6A KO cells, which may express a truncated lower molecular weight variant. These cell lines were also used for IF. In addition, antibodies were evaluated by IP using HAP1 cell lysates (1 mg protein). All experiments were performed according to YCharOS standard protocols (see reference).

In WB (1:1000 antibody dilution), NBP1-33110 detects a band at the expected size (~24 kDa) in WT HAP1 cell lysate with no corresponding band in lysate from Rab6A KO cells. In addition several weak background bands are detected in both lysates.

In IP, the antibody (2 µg) precipitates Rab6A, as detected by WB using the Rab6A-specific antibody NBP3-13701. However, the band in the IP fraction appears comparable in intensity to the band in starting material and unbound fraction, indicating some but incomplete immunoprecipitation.

In IF, the antibody (1:800 dilution) resulted in a good staining of HAP1 WT with little background in Rab6A KO cells.

We highly encourage other scientists to share their observations.