Rab27A, Proteintech, 66058-1-Ig

Our team performed a comprehensive review of the scientific literature to identify studies using antibodies against human Rab27A and Rab27B, followed by extraction and evaluation of available data according to pAbmAbs rating criteria.

YCharOS conducted a systematic head-to-head comparison of 14 commercially available antibodies against Rab27A and 7 against Rab27B across multiple applications

Antibodies were tested by WB using lysates (40 µg total protein) from U-87 MG WT cells and cells with siRNA-mediated knockdown (KD) of Rab27A or Rab27B. The same cell lines were used for IF testing. In addition, antibodies were evaluated by IP using HAP1 (Rab27A) or U-87 MG (Rab27B) cell lysates (1 mg), following YCharOS standard protocols (see reference).

In Western blot (1:1000 antibody dilution), 66058-1-Ig detects a band at the expected size (~24 kDa) in WT U-87 MG lysate. In lysate from Rab27B KD cells, a band of comparable intensity is observed. In Rab27A cell lysate is observed a weaker band with a possible slight upward shift in size. Together, these results indicate that the antibody detects both Rab27A and Rab27B.

In IP, the antibody (2 µg) does not enrich Rab27A following precipitation, as detected by WB using the Rab27A-specific antibody ab313575. Rab27A in the starting material, unbound fraction, and precipitate was assessed.

Antibody 66058-1-Ig was not tested in IF.

We highly encourage other scientists to share their observations.