Rab27A, Bio-Techne, MAB7245

Our team performed a comprehensive review of the scientific literature to identify studies using antibodies against human Rab27A and Rab27B, followed by extraction and evaluation of available data according to pAbmAbs rating criteria.

YCharOS conducted a systematic head-to-head comparison of 14 commercially available antibodies against Rab27A and 7 against Rab27B across multiple applications

Antibodies were tested by WB using lysates (40 µg total protein) from U-87 MG WT cells and cells with siRNA-mediated knockdown (KD) of Rab27A or Rab27B. The same cell lines were used for IF testing. In addition, antibodies were evaluated by IP using HAP1 (Rab27A) or U-87 MG (Rab27B) cell lysates (1 mg), following YCharOS standard protocols (see reference).

In WB (1:200 antibody dilution), MAB7245 detects a band at the expected size (~24 kDa) in WT U-87 MG lysate, with no corresponding band in lysates from Rab27A KD cells. In addition, two non-specific weaker bands of higher Mw are observed in both lysates.

In IP, the antibody (2 µg) does not enrich Rab27A following precipitation, as detected by WB using the Rab27A-specific antibody 95394 from Cell Signaling Technology. Rab27A in the starting material, unbound fraction, and precipitate was assessed.

In IF, the antibody (1:150 antibody dilution) resulted in a strong to medium signal from U-87 MG WT and with no background signal in Rab27A KD cells.

We highly encourage other scientists to share their observations.