Tuberin, Cell Signaling Technology, 4308

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Tuberin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Tuberin encoded by TSC2.

The collection of antibodies was tested using HAP1 WT and TSC2 KO cells in the applications WB (WT and KO cell lysates/100 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 4308 detected a band of appr. 200 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to band in input extract (10% of starting material) and no band was detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:500 ab dilution) an intense staining was observed in WT cells compared to KO cells showing no background.

We highly encourage other scientists to share their observations.