Tuberin, Thermo Fisher Scientific, 37-0500

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Tuberin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Tuberin encoded by TSC2.

The collection of antibodies was tested using HAP1 WT and TSC2 KO cells in the applications WB (WT and KO cell lysates/100 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:250 ab dilution) 37-0500 detected a band of appr. 200 kDa in WT cell lysate with a faint corresponding band observed in KO cell lysate. Stronger bans of both higher and lower molecular weight were observed in both genotypes. A band was detected in immunodepleted fraction (10% unbound fraction) and no band showed for IP (2 µg Ab used). In IF (1:500 ab dilution), comparable staining intensity was observed in both WT and KO cells.

We highly encourage other scientists to share their observations.