Hamartin, Cell Signaling Technology, 6935

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Hamartin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 5 commercial research antibodies against human Hamartin encoded by TSC1.

The collection of antibodies was tested using HAP1 WT and TSC1 KO cells in the applications WB (WT and KO cell lysates/100 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) 6935 detected a band of appr. 135 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Bands of lower molecular weight was observed in both genotypes. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to band in input extract (10% of starting material) and no band was detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:500 ab dilution), comparable staining intensity was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.