TRIM21, Cell Signaling Technology, 92043

I am sharing a pAbmAbs generated literature review of KO-validated antibodies, here citing Park et al., Nature, 2020.

Park et al. validated the TRIM21 antibody 92043 for the detection of endogenous human TRIM21 by Western blotting using control and CRISPR-generated TRIM21-knockout (KO) H2009 non-small-cell lung cancer cells. TRIM21 was detected in control cells, whereas no corresponding signal was observed in TRIM21-KO cells (Extended Data Figure 7e), supporting the specificity of the antibody for endogenous TRIM21. Uncropped images in Supplementary information support this.

Based on the available validation data, we have assigned this antibody a five-star rating, but we highly encourage other scientists to share their observations and experiences with this antibody.

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human The tripartite motif containing-21(TRIM21) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human The tripartite motif containing-21encoded by the TRIM21 gene.

The collection of antibodies was tested using A549 WT and TRIM21 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 ab dilution) 92043 detected a band of appr. 50 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Bands of higher molecular weight was observed in both genotypes. A band was detected following IP (2 µg Ab used), this band was more intense compared to band in input extract (5% of starting material) and in. immunodepleted extract (5% of total unbound fraction). In IF (1:100 ab dilution), a more intense staining was observed in WT cells compared to KO cells showing background.

We highly encourage other scientists to share their observations.