TRIM21, Cell Signaling Technology, 74400

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human The tripartite motif containing-21(TRIM21) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human The tripartite motif containing-21encoded by the TRIM21 gene.

The collection of antibodies was tested using A549 WT and TRIM21 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 ab dilution) 74400 detected a band of appr. 50 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was detected following IP (2 µg Ab used), this band was more intense compared to band in input extract (5% of starting material). No band was also detected in immunodepleted extract (5% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:500 ab dilution), a more intense staining was observed in WT cells compared to KO cells showing some background.

We highly encourage other scientists to share their observations.