TMEM175, Thermo Fisher Scientific, 703805**

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Endosomal/lysosomal proton channel TMEM175 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human Endosomal/lysosomal proton channel TMEM175 encoded by TMEM175. The collection of antibodies was tested using HeLa WT and TMEM175 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 703805 did not detected a band specific to WT cell lysate. Only a heavy chain band was detected following IP (2 µg Ab used).

In IF (1:100 ab dilution), a more intense staining was observed in WT cells with some background observed in KO cells.

We highly encourage other scientists to share their observations.