TMEM175, Proteintech, 68172-1-Ig

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Endosomal/lysosomal proton channel TMEM175 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human Endosomal/lysosomal proton channel TMEM175 encoded by TMEM175. The collection of antibodies was tested using HeLa WT and TMEM175 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:5000 ab dilution) 68172-1-Ig detected an intense band of appr. 42 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Bands of higher molecular weight was observed in both genotypes. No band was detected following IP (2 µg Ab used).

In IF (1:500 ab dilution), comparable staining intensities was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.