TMEM175, Abcam, ab309572

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Endosomal/lysosomal proton channel TMEM175 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human Endosomal/lysosomal proton channel TMEM175 encoded by TMEM175. The collection of antibodies was tested using HeLa WT and TMEM175 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab309572 detected a strong band of appr. 42 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Fainter bands of higher molecular weight was observed in both genotypes. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to bands in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction), showing that the antibody does participate most of the antigen; however, not all.

In IF (1:500 ab dilution) a more intense staining was observed in WT cells with some background observed in KO cells.

We highly encourage other scientists to share their observations.