TICAM1, Thermo Fisher Scientific, MA5-56479

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human TIR domain–containing adapter molecule 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human TIR domain–containing adapter molecule 1 encoded by TICAM1.

The collection of antibodies was tested using U-87 MG WT and TICAM1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 ab dilution) MA5-56479 detected a band of appr. 100 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Only a heavy chain band was detected following IP (2 µg Ab used). In IF (1:500 ab dilution), comparable staining intensity was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.