TICAM1, Proteintech, 23288-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human TIR domain–containing adapter molecule 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human TIR domain–containing adapter molecule 1 encoded by TICAM1.

The collection of antibodies was tested using U-87 MG WT and TICAM1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 23288-1-AP did not detect a band specific to the WT cell lysate. Only a heavy chain band was detected following IP (2 µg Ab used).

In IF (1:150 ab dilution) comparable staining intensity was observed in both KO and WT cells, with few KO cells having more intense staining compared to WT cells.

We highly encourage other scientists to share their observations.