TICAM1, ABclonal, A13605

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human TIR domain–containing adapter molecule 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human TIR domain–containing adapter molecule 1 encoded by TICAM1.

The collection of antibodies was tested using U-87 MG WT and TICAM1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) A13605 detected a band of appr. 100 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Bands of both higher and lower molecular weight was observed in both genotypes. A band was detected following IP (2 µg Ab used), this band had same intensity compared to bands in input extract (6% of starting material) and immunodepleted extract (6% of total unbound fraction), showing that the antibody does not deplete the antigen from cell lysate. In IF (1:300 ab dilution), a more intense stain was observed in WT cells compared to KO showing some background.

We highly encourage other scientists to share their observations.