TIA1, Thermo Fisher Scientific, MA5-26474

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human T-cell intracellular antigen (TIA1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human T-cell intracellular antigen encoded by TIA1.

The collection of antibodies was tested using HAP1 WT and TIA1KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 ab dilution) MA5-26474 detected a band of appr. 45 kDa in both KO and WT cell lysate. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to band in input extract (4% of starting material) and no band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:1000 ab dilution), a more intense staining was observed in WT cells with some background in KO cells.

We highly encourage other scientists to share their observations.