TIA1, Bio-Techne, NBP3-13791

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human T-cell intracellular antigen (TIA1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human T-cell intracellular antigen encoded by TIA1.

The collection of antibodies was tested using HAP1 WT and TIA1KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) NBP3-13791 detected a smear in both genotypes with no band being specific to WT cell lysate. Only the unbound fraction was showed for NBP2-53336, having the same intensity as input extract (4% of starting material). In IF (1:200 ab dilution) most WT and KO cells showed same staining intensity with few WT cells have more intense staining.

We highly encourage other scientists to share their observations.